The Primer Generator - Manuscript

The Primer Generator

Original nucleotide sequence	GAGGCCTCTCGCTTG
Original protein sequence	EASRL
Desired amino acid sequence	EARRL
Use of restriction enzymes with site size less than 5 bp	No
Use of only most common restriction enzymes	No
Maximum number of substitutions compared to original sequence	3

The following primers have been generated:

Click on enzyme name to access corresponding entry in The Restriction Enzyme Database (REBASE).
Positions of cut sites are calculated relative to the beginning of the primer.
Differences between primer sequence and original nucleotide sequence are bolded