The Primer Generator - Frequently Used Enzymes


Listed below are the restriction endonucleases that The Primer Generator uses to compute restriction sites for potential primers when "Yes" is chosen for the setting "Limit to the most frequently used enzymes". In the authors' experience these enzymes are commonly used in laboratories and are therefore likely to be easily accessible for experiments; if you feel that any other enzymes should be added to this list please let us know.
AccI AflIII ApaLI AscI AvaI
BamHI BhlI BglII BssHII BstEII
BstXI ClaI DraI EcoRI EcoRV
FokI HaeIII HindIII KpnI MluI
NaeI NarI NcoI NdeI NheI
NotI NsiI PacI PmeI PstI
PvuI PvuII SacI SacII SalI
ScaI SmaI SpeI SphI StuI
XbaI XhoI XmaI


Authors:
Joseph Lawler
Alexander Turchin